BIODIVERSITY AND ENZYME ACTIVITY OF INDIGENOUS CELLULOLYTIC AND AMYLOLYTIC BACTERIAS IN DECAYED MANGROVE STEM WASTE PRODUCT AT WAAI SEASHORE, AMBON ISLAND

Abstract

Mangrove (Sonneratia spp.) could be found at Waai seashore, Ambon Island. The remainder of the mangrove stem will be decayed and become the waste product. Some indigenous bacteria species that live in the decayed mangrove stem waste product have cellulolytic and amylolytic characters. The objectives of this research were to: 1) identify and determine the cellulolytic bacteria species; 2) identify and determine the amylolytic bacteria spesies; 3) determine the cellulolytic bacteria species that have the highest cellulase activity; 4) determine the amylolytic bacteria species that have the highest amylase activity. This research was conduct at the Microbiology Laboratory-Biology Departement-State University of Malang, Microbiology Laboratory-Faculty of Medicine-Brawijaya University, Chemistry Laboratory-Muhammadiyah Malang University. Twenty five grams sample were ground and diluted in 225 ml nutrient broth to get 10-1 suspension. Then the suspension were diluted gradually until 10-10. The suspension were inoculated on nutrient agar medium 0.1 ml each, and incubated in 370C during 1x24 hours. Each bacteria colonies were isolated and determine to know which one were the cellulolytic and the amylolytic bacteria. The bacteria isolates were identify. Afterward the cellulase activity as well as the amylase activity were analyzed. The research results shows that: 1) there are 4 cellulolytic bacteria spesies, i.e: Micrococcus luteus, Bacillus pumilus, Planococcus citreus, and Bacillus cereus; 2) there are 4 amylolytic bacteria species, i.e: Bacillus firmus, Nitrobacter sp., Bacillus mycoides, and Pseudomonas aeruginosa; 3) Bacillus cereus has the highest cellulase activity; 4) Nitrobacter sp. has the highest amylase activity. Keywords : biodiversity, cellulolytic bacteria, amylolytic bacteria, decayed mangrove stem waste product.