Metabolic Profiling of the Mesenchymal Stem Cells’ Secretome

Abstract

Human 'multipotent mesenchymal stromal cells' (hMSCs) are popular cells in human regenerative medicine due to their ability to renew themselves and differentiate into various specialized cell types under specific physiological or experimental conditions. HMSCs secrete a broad spectrum of components including proteins and metabolites that represent significant effects on the cells in their neighborhood. Furthermore, it assists to characterize them. The therapeutic effects of hMSCs were thought to be due to their multipotent characterization and their ability to engraft and differentiate at the site of injury. However, recent studies have revealed the fact that a secretome plays important role in the therapeutic potential of hMSCs. Here, we outline the decoding of the metabolome for hMSCs secretome through metabolic profiling by application of MALDI-TOF-MS which could contribute to a better understanding of the therapeutic effects associated with hMSCs and their characterization.