Abstract
In recent years, microRNAs (miRNA) have been demonstrated to be present in body fluids and may therefore serve as diagnostic markers for diseases. By characterizing miRNA profiles in plasma, a miRNA signature may potentially be developed as a diagnostic and risk assessment tool for particular (patho)physiological states. This chapter describes the isolation, purification, identification, and sequencing of human plasma miRNAs.
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van der Eerden, B. C., Alves, R. D., Kockx, C. E., Ozgur, Z., Schreuders-Koedam, M., van de Peppel, J., … van Leeuwen, J. P. (2015). Identification of microRNAs in human plasma. Methods in Molecular Biology, 1226, 71–85. https://doi.org/10.1007/978-1-4939-1619-1_7
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