Optogenetics uses light to manipulate protein localization or activity from subcellular to supra-cellular level with unprecedented spatiotemporal resolution. We used it to control the activity of the Cdc42 Rho GTPase, a major regulator of actin polymerization and cell polarity. In this chapter, we describe how to trigger and guide cell migration using optogenetics as a way to mimic EMT in an artificial yet highly controllable fashion.
CITATION STYLE
Valon, L., & de Beco, S. (2020). Control of cell migration using optogenetics. In Methods in Molecular Biology (Vol. 2179, pp. 415–425). Humana Press Inc. https://doi.org/10.1007/978-1-0716-0779-4_29
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