Field-amplified sample stacking β-cyclodextrin modified capillary electrophoresis for quantitative determination of diastereomeric saponins

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Abstract

Successful simultaneous diastereomeric separation and sensitive determination of two pairs of triterpenoidal saponins have been achieved by capillary electrophoresis (CE) using β-cyclodextrin (β-CD) as a stereoselective agent to cooperate with borate complexation. A usual technique for isolation and group separation of saponins was developed as an appropriate purification step prior to the determination of individual saponins by CE. Soyasaponin I (S1), azukisaponin V (S2), bersimoside I (S3) and bersimoside II (S4) could be well separated within 14 min in a fused-silica capillary (60 cm long to the detector with an additional 10 cm to the cathode; 75 μm i.d.). The background electrolyte was borate buffer (80 mM, pH 10), containing 24 mM β-CD. The separation voltage was 14 kV with a detection wavelength of 195 nm. The sample was electrokinetically injected using a voltage of 16 kV for 12 s. Methanol (70%) was used as the diluent for field-amplified sample stacking after hydrodynamic injection of short water plug (5 cm, 4 s). The method was partially validated for linearity, repeatability, reproducibility, limits of detection and limits of quantification. The correlation coefficients of the calibration curves were all >0.998, and the recoveries were from 98.23 to 96.21%.

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Emara, S., Masujima, T., Zarad, W., Mohamed, K., Kamal, M., Fouad, M., & EL-Bagary, R. (2014). Field-amplified sample stacking β-cyclodextrin modified capillary electrophoresis for quantitative determination of diastereomeric saponins. Journal of Chromatographic Science, 52(10), 1308–1316. https://doi.org/10.1093/chromsci/bmt169

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