Hepatoprotective activity from ethanol extract of pugun tano’s leave (Curanga Fel-Terrae [lour.] merr.)

Abstract

Objective: The aim of this study is to observe the activity of ethanol extract of Curanga fel-terrae leave in preventing the damaged of liver which is induced by high dose of paracetamol. Methods: This research was conducted using Wistar rat divided into six groups. Group 1 was the normal group. Groups 2, 3, 4, 5, and 6 received carboxymethyl cellulose-Na 0.5% (negative control), C. fel-terrae ethanolic extract (CFEE) at the doses of 125, 250 and 500 mg/kg, catechin 2 mg/kg (positive control), respectively, during 7 days continued and followed by given paracetamol dose of 2.5 g/kg 8 hrs. After that, hepatoprotective activity was carried out toward parameter of aspartate aminotransferase (AST), alanine aminotransferase (ALT) as well as histopathology of the liver. Results: The results showed that high dose paracetamol dose of 2.5g/kg bw can cause liver damaged which can be seen by the increasing of the level of AST and ALT compared to the normal group (p<0.05). The usage of three doses of CFEE for 7 days showed the prevention of the increasing of the level of AST and ALT compared to negative control group (p<0.05). Furthermore, the histopathology study revealed that the three doses of extract could protect the liver. Conclusion: The CFEE at the doses of 125, 250 and 500 mg/kg bw which was given for 7 days can prevent the liver from the damage caused by a high dose of paracetamol.