Widespread bacterial resistance to antibiotics is a significant public health concern. To remain a step ahead of evolving bacteria, new methods to study resistance to antibacterials and to uncover novel antibiotics that evade resistance are urgently needed. Herein, microarray-based methods that have been developed to study aminoglycoside modification by resistance-causing enzymes are reviewed. These arrays can also be used to study the binding of aminoglycoside antibiotics to a mimic of their therapeutic target, the rRNA aminoacyl site (A-site), and how modification by resistance-causing enzymes affects their abilities to bind RNA. © 2012 Springer Science+Business Media, LLC.
CITATION STYLE
Disney, M. D. (2012). Studying modification of aminoglycoside antibiotics by resistance-causing enzymes via microarray. Methods in Molecular Biology, 808, 303–320. https://doi.org/10.1007/978-1-61779-373-8_21
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