Nanostructural materials increase mineralization in bone cells and affect gene expression through miRNA regulation

Abstract

We report that several nanomaterials induced enhanced mineralization (increased numbers and larger areas of mineral nests) in MC3T3-E1 bone cells, with the highest response being induced by silver nanoparticles (AgNPs). We demonstrate that AgNPs altered microRNA expression resulting in specific gene expression associated with bone formation. We suggest that the identified essential transcriptional factors and bone morphogenetic proteins play an important role in activation of the process of mineralization in bone cells exposed to AgNPs. © 2011 Foundation for Cellular and Molecular Medicine/Blackwell Publishing Ltd.