Effect of Non-Native Endophytic Bacteria on Oat (Avena sativa L.) Growth

Abstract

Endophytic bacteria are known to influence vital activities of host plants. Endophytes can promote plant growth and provide a defense response against pathogens. The use of endophytes in crop production has the potential to reduce the application of fertilizer and pesticide input and thus improve the sustainability of crop production. In this study, we investigated the effects of seed inoculation with non-native endophytic bacteria, harvested from Brassica carinata, on oat (Avena sativa L.) growth with root vigor assays and greenhouse experiments. For root vigor assay experiments, seeds of two different oat cultivars were treated with 16 endophytic bacteria previously shown to promote growth benefits on multiple crop species. For the greenhouse experiments, the effect of seed inoculation with bacterial isolates was evaluated on ten oat cultivars at two fertilization levels. The root vigor assay showed that multiple isolates, including Bacillus licheniformis, Enterobacter kobei, B. halotolerans, B. cereus, B. aryabhattai, and Lysinibacillus fusiformis, had a positive effect on seedling root growth in one of the two oat cultivars. In the other cultivar, the bacterial isolates had either no effect or a negative effect on root growth. Greenhouse studies showed that the magnitude and direction of the effect of bacterial inoculation on oat growth varied with fertilization levels, bacterial strain, and oat cultivar. However, we identified two cultivars that were more responsive to bacterial inoculation than the others and for which bacterial inoculation of seed resulted in enhanced growth in several traits under both reduced and full nitrogen levels, and this response was observed for the two isolates tested. Our results show that inoculating oat seeds with non-native bacterial endophytes can promote root and shoot growth in oats. Developing biofertilizers that are effective across crop species, crop cultivars, and environmental conditions may be possible if cultivars are selected for their responsiveness across multiple bacterial isolates and in multiple growing environments. Overall, this study indicates that non-native endophytes could be considered for the development of biofertilizers with effectiveness across crop species.