Caracterização filogenética de isolados de Beauveria bassiana originados de diferentes insetos hospedeiros

Abstract

This study aimed to evaluate genetic variability using the internal transcribed spacer (ITS) region of ribosomal DNA (rDNA) and the phylogeny of five Beauveria bassiana: IPA145, IPA148, IPA223, IPA225 and IPA226, obtained from different hosts and geographical origin from the Biological Control Laboratory of the Agronomic Institute of Pernambuco - IPA. The ITS region was amplified using primers ITS1 and ITS4, where an amplicon with average size of 800 bp was observed and sequenced. The DNA sequences of Beauveria isolates were compared with sequences in the database (GenBank). The results show that all isolates, except IPA145, were presented as a monophyletic group. IPA145 showed a genetic identity of 99% with Cordyceps bassiana and B. bassiana. IPA148 was the most genetically distant from the others, with less than 40% similarity. Most of the Beauveria isolates studied proved to be phylogenetically distinct.