Although proteins can be artificially improved by random insertion and deletion mutagenesis methods, these procedures are technically difficult. Here we describe a simple method called random insertional-deletional strand exchange mutagenesis (RAISE). This method is based on gene shuffling and can be used to introduce a wide variety of insertions, deletions, and substitutions. RAISE involves three steps: DNA fragmentation, attachment of a random short sequence, and reconstruction. This yields unique mutants and can be a powerful technique for protein engineering. © 2014 Springer Science+Business Media New York.
CITATION STYLE
Fujii, R., Kitaoka, M., & Hayashi, K. (2014). Random insertional-deletional strand exchange mutagenesis (RAISE): A simple method for generating random insertion and deletion mutations. Methods in Molecular Biology, 1179, 151–158. https://doi.org/10.1007/978-1-4939-1053-3_10
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