Analysis of the human genome sequence has identified thousands of putative genes with unknown function; therefore, a new tool allowing for rapid identification of gene functions is needed. Reverse transfection microarray technology, which turns a DNA microarray into a cell-based microarray, has emerged for simultaneously studying the function of many genes. Since the initial demonstration in 2001, many variations have surfaced, making the technology more versatile for a broad range of applications. We have developed a protocol to make ready-to-transfect DNA microarrays in a 96-well microplate for cotransfection of two plasmids into HEK293T cells. This cell-based microarray in a microplate may be used for screening hundreds of analytes against multiple protein targets in parallel, providing a powerful tool for functional genomics and drug discovery.
CITATION STYLE
Soni, M., & Lai, F. (2011). Cell-based co-transfection microarrays for use with HEK293T cells on a poly D-Lysine-coated polystyrene microplate. In Methods in Molecular Biology (Vol. 706, pp. 13–25). Humana Press Inc. https://doi.org/10.1007/978-1-61737-970-3_2
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