Gluconeogenesis and Glycolysis in Isolated Perfused Liver of the Eel

Abstract

A method for perfusion of isolated eel (Anguilla japonica) liver is described. The perfusion medium employed was Krebs-Ringer bicarbonate without oxygen carriers such as red cells or fluoro carbon and albumin. The rate of gluconeogenesis in excess of lactate was almost the same as that of mammals. Insulin inhibited glycogenolysis of liver glycogen. Epinephrine and 3′, 5′,-c-AMP stimulated glycogenolysis. Activities of aspartate transaminase and alanine transaminase were maintained low during perfusion. Oxygen consumption was 1/5 to 1/7 of that in the perfused rat liver. These results indicate that our perfusion technique provides a useful method for studies of metabolic regulation of eel liver. © 1975, The Japanese Society of Fisheries Science. All rights reserved.