Detection of immunomagnetically captured escherichia coli o157:h7 by antibody-conjugated alkaline phosphatase

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Abstract

A rapid and sensitive detection process for Escherichia coli O157:H7 was developed using alkaline phosphatase (APase)-labeled anti-E. coli O157 antibodies to tag the targeted bacteria. Immunomagnetic beads or antibody-labeled streptavidin-coated magnetic beads were then used to capture the APase-tagged E. coli. Immunomagnetically captured bacteria were washed and distributed into microplates or optical cuvettes. The enzyme-catalyzed hydrolysis of p-nitro-phenol phosphate in alkaline solutions was then followed. Less than 1000 cfu/ml of E. coli O157:H7 could be detected. This approach was applied to detect the bacteria artificially spiked in beef hamburgers. Less than 1 cfu/g of E. coli O157:H7 produced a significant response after cultural enrichment for 4-6 h at 37°C.

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APA

Tu, S. I., Patterson, D., Briggs, C., Irwin, P., & Yu, L. (2001). Detection of immunomagnetically captured escherichia coli o157:h7 by antibody-conjugated alkaline phosphatase. Journal of Industrial Microbiology and Biotechnology, 26(6), 345–349. https://doi.org/10.1038/sj.jim.7000141

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