High prevalence of fecal carriage of extended-spectrum β-lactamase/AmpC-producing Enterobacteriaceae in cats and dogs

Abstract

Extended-spectrum-β-lactamase (ESBL)/AmpC producing Enterobacteriaceae have been reported worldwide obtained from humans, food-producing animals, companion animals, and environmental sources. However, fecal carriage of ESBL/AmpC producing Enterobacteriaceae in healthy companion animals is limited. This prevalence of ESBL/AmpC encoding genes in healthy cats and dogs, and cats and dogs with diarrhea. Twenty group were cultured on MacConkey agar supplemented with 1 mg/L cefotaxime and in LB-enrichment broth mg/L cefotaxime, which was subsequently inoculated on MacConkey agar supplemented with 1 mg/L cefotaxime. were identified using the Check-Points CT103 micro array kit and subsequently by sequencing analysis. Chromosomal promoter mutations were detected by PCR and sequencing analysis. From the healthy and diarrheic were positive for Escherichia coli with reduced susceptibility for cefotaxime. From the healthy and diarrheic prevalence was respectively 0 and 25%. One diarrheic cat was positive for both reduced susceptible ESBL/AmpC genes found in this study were mainly blaCTX-M-1, but also blaCTX-M-14, blaCTX-M-15, bla were detected. This pilot study showed that the prevalence of ESBL/AmpC producing Enterobacteriaceae dogs, and diarrheic cats was relatively high. Furthermore, the genes found were similar to those found in food-producing animal origin. However, since the size of this study was relatively small, extrapolation of the population of cats and dogs should be done with great care. © 2013 Hordijk, Schoormans, Kwakernaak, Duim, Broens, Dierikx, Mevius and Wagenaar.