Pulse-chase radiolabeling of cells with radioactive amino acids is a common method for studying the biosynthesis of proteins. The labeled proteins can then be immunoprecipitated and analyzed by electrophoresis and gel imaging techniques. This chapter presents a protocol for the biosynthetic labeling and immunoprecipitation of pancreatic islet proteins which are known to be affected in disorders such as diabetes, obesity, and metabolic syndrome.
CITATION STYLE
Guest, P. C. (2018). Pulse-chase biosynthetic radiolabeling of pancreatic islets to measure beta cell function. In Methods in Molecular Biology (Vol. 1735, pp. 331–341). Humana Press Inc. https://doi.org/10.1007/978-1-4939-7614-0_22
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