Antibody-dependent cell-mediated cytotoxicity (ADCC) is a relevant characteristic to measure for a number of therapeutic monoclonal antibodies (mAbs) under development. ADCC is a mechanism by which antibody-opsonized, infected, or cancerous cells are destroyed by FcγRIII (CD16)-expressing effector cells. Here we describe three methods that can be used to quantify the ADCC activity of mAbs by measuring distinct aspects of the ADCC mechanism. © 2014 Springer Science+Business Media New York.
CITATION STYLE
Miller, A. S., Tejada, M. L., & Gazzano-Santoro, H. (2014). Methods for measuring antibody-dependent cell-mediated cytotoxicity in vitro. Methods in Molecular Biology, 1134, 59–65. https://doi.org/10.1007/978-1-4939-0326-9_5
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