Upregulation of HSP72 in Reloading Rat Soleus Muscle after Prolonged Hindlimb Unloading

Abstract

To clarify the changes of heat shock protein (HSP) 72 in the rat soleus muscle after hind-limb unloading (HU) and during reloading, 7-week-old male Wistar rats were hind-limb-suspended for 9 weeks, thereafter ambulatory recovery was permitted for 8 weeks. The body and absolute soleus weights were significantly lower in the HU than in the age-matched control group after HU and during reloading. The soleus weight relative to body weight was also significantly lower in the HU than in the age-matched control group at the end of 9 weeks of suspension, but returned to the control level after 2 weeks of reloading. The HSP72 content decreased to 38% of the control level after HU and conversely increased to 165 and 175% of the control level after 2 and 4 weeks of reloading, respectively. The HSP72 content returned to the control level after 8 weeks of reloading. Thus our results showed that the expression of HSP72 was down-regulated by HU and upregulated temporally over the level of the control during the reloading period, and they suggested that these down- and up-regulations of HSP72 may be related to many factors including mechanical stress or load applied to the muscle.