Detoxification of afiatoxin B1 and change in microflora pattern by probiotic in vitro fermentation of broiler feed

Abstract

The aim of this study was to determine the influence of spontaneous fermentation and that with the use of probiotic bacteria and yeast on afiatoxin B, concentration and the microflora pattern during fermentation. The probiotic preparation used contained bacteria resistant to gastric juice and bile: Lactobacillus paracasei LOCK 0920, Lactobacillus brevis LOCK 0944, Lactobacillus plantarum LOCK 0945, as well as live yeasts Saccharomyces cerevisiae LOCK 0140 of high fermenting capacity. After 6-h fermentation with the probiotic, in feed mixture with a low concentration of afiatoxin B, (1 mg/kg), the amount of afiatoxin Bj decreased by 55%. In the case of a high concentration (5 mg/kg) the decrease in afiatoxin B, was about 39%. This tendency was sustained during the following hours of incubation (12* and 24* h). The application of probiotic bacteria and yeasts resulted in the reduction of aerobic spore forming bacteria.