In label-free biosensing, a continuous improvement of the limit of detection is necessary to resolve the small change of the surface refractive index produced by interacting biomolecules at a very small concentration. In the present work, optical sensors based on one-dimensional photonic crystals supporting Bloch surface waves are proposed and adopted for label-free optical biosensing. We describe the implementation of an angularly resolved ellipsometric optical sensing scheme based on Bloch surface waves sustained by tantala/silica multilayers. The angular operation is obtained using a focused beam at fixed wavelength and detection of the angular reflectance spectrum by means of an array detector. The results show that the experimental limit of detection for a particular photonic crystal design is 6.5×10−7 refractive index units (RIU)/Hz1/2 and further decrease could be obtained. For the first time, we report on the practical application of this technique to a cancer biomarker protocol that aims at the detection of a specific glycoprotein (angiopoietin 2) involved in angiogenesis and inflammation processes.
CITATION STYLE
Sinibaldi, A., Anopchenko, A., Rizzo, R., Danz, N., Munzert, P., Rivolo, P., … Michelotti, F. (2015). Angularly resolved ellipsometric optical biosensing by means of Bloch surface waves. Analytical and Bioanalytical Chemistry, 407(14), 3965–3974. https://doi.org/10.1007/s00216-015-8591-8
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