A fluorescence polarization assay can be used to evaluate the strength of a protein-protein interaction. A green fluorescent protein variant is fused to one of the protein partners. The formation of a complex is then deduced from an increase in fluorescence polarization, and the equilibrium dissociation constant of the complex is determined in a homogeneous aqueous environment. The assay is demonstrated by using the interaction of the S-protein and S-peptide fragments of ribonuclease A as a case study.
CITATION STYLE
Park, S. H., & Raines, R. T. (2004). Fluorescence polarization assay to quantify protein-protein interactions. Methods in Molecular Biology (Clifton, N.J.). https://doi.org/10.1385/1-59259-762-9:161
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