MicroRNAs (miRNAs) are single-stranded, small RNA molecules of 21-23 nucleotides that are primarily involved in the regulation of gene expression. A number of recent studies have shown that miRNAs play a vital role in signaling pathways important for human oncogenesis and may hold promise as potential biomarkers for cancer diagnosis, prognosis, and therapeutics. Microarray-based expression analysis is an emerging and powerful strategy for initially identifying candidate miRNAs, which can then be correlated to specific biological process such as carcinogenesis, and eventually developed as a molecular signature for a disease state. This chapter presents a protocol for miRNA microarray profiling using the Agilent platform, which is one of the most widely utilized technologies currently available. © 2011 Springer Science+Business Media, LLC.
CITATION STYLE
Ranade, A. R., & Weiss, G. J. (2011). Methods for MicroRNA microarray profiling. Methods in Molecular Biology, 700, 145–152. https://doi.org/10.1007/978-1-61737-954-3_10
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