Flow cytometric determinations of glycogen content of yeast during fermentation

Abstract

Using flow cytometric analysis, the rapid detection of glycogen content of yeast cells in process is possible. Glycogen is a sensitive parameter which can express the physiological state of the yeast cells. A procedure was developed to stain and measure glycogen in yeast cells using flow cytometry. Glycogen content of lager yeast cells was tested after 1 and 15 h of nutrient limitation.