Pengaruh Penambahan Gliserol pada Media Perbanyakan terhadap Daya Simpan Biofungisida Trichoderma

Abstract

The storage longevity of biofungicide containing Trichoderma virens and T. amazonicum to controll the whithe root diseases (WRD) in rubber plants is important to know to remain effective when applied. The composition of the multiplication medium in the T. virens dan and T. amazonicum biofungicide can affect the longevity and viability of conidia during storage. The objective of the research was to determine the effect of glycerol addition in the multiplication medium of T. virens and T. amazonicum biofungicide during storage. The research was conducted at the Laboratory of Plant Protection, Indonesian Industrial and Beverage Crops Research Institute (IIBCRI), Sukabumi, from January to July 2014. The experiment used a completely randomized design with 8 treatments and 3 replications. The biofungicidal formula consisted of: (1) glycerol addition (0%, 3%, 6%, and 9%) in the multiplication medium of T. virens and (2) glycerol addition (0%, 3%, 6%, and 9%) in the multiplication medium of T. amazonicum. Each multiplication was mixed with a carrier (talc), dried, then packed in a plastic bag and stored for 4 months. Observations were carried out each month, including: the amount of conidia, Trichoderma sp. population, and water content. The results showed that the addition of glycerol to multiplication medium was able to maintain the viability of T. virens and T. amazonicum and the storage longevity of the biofungicide. The addition of 6% to 9% glycerol in the multiplication medium of T. virens and T. amazonicum showed the highest concentration, yielding conidia up to 7.98 x 10 7 – 8.59 x 10 7 conidia/g and population abundance of 11.67 x 10 3 – 14.67 x 10 3 cfu/g in biofungicide stored for 4 months.