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The basic polymerase chain reaction (PCR)

Cold Spring Harbor Protocols (2018) 2018(5) 338-345
DOI: 10.1101/pdb.prot095117
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Abstract

This protocol describes the reagents and procedures required to amplify a segment of double-stranded DNA in a chain reaction catalyzed by a thermostable DNA polymerase. It is the foundation for all subsequent variations of the polymerase chain reaction (PCR).

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APA

Green, M. R., & Sambrook, J. (2018). The basic polymerase chain reaction (PCR). Cold Spring Harbor Protocols, 2018(5), 338–345. https://doi.org/10.1101/pdb.prot095117

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