G protein-coupled receptors (GPCRs) are notable targets of basic therapeutics. Many screening methods have been established to identify novel agents for GPCR signaling in a high-throughput manner. However, information related to the temporal reaction of GPCR with specific ligands remains poor. We recently developed a bioluminescence method for the quantitative detection of the interaction between GPCR and β-arrestin using split luciferase complementation. To monitor time-course variation of the interactions, a new imaging system contributes to the accurate evaluation of drugs for GPCRs in a high-throughput manner.
CITATION STYLE
Hattori, M., & Ozawa, T. (2016). Live cell bioluminescence imaging in temporal reaction of g protein-coupled receptor for high-throughput screening and analysis. In Methods in Molecular Biology (Vol. 1461, pp. 195–202). Humana Press Inc. https://doi.org/10.1007/978-1-4939-3813-1_16
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