Characterization of protein structure modifications is an important field in mass spectrometry (MS)-based proteomics. Here, we describe a process to quickly and reliably identify a mass change in a targeted protein sequence by top-down mass spectrometry (TD MS) using electron transfer dissociation (ETD). The step-by-step procedure describes how to develop a TD MS method for data acquisition as well as the data analysis process. The described TD MS workflow utilizes diagnostic ions to characterize an unknown sample in a few hours.
CITATION STYLE
Coelho Graça, D., Hartmer, R., Jabs, W., Scherl, A., Clerici, L., Samii, K., … Lescuyer, P. (2019). Detection of proteoforms using top-down mass spectrometry and diagnostic ions. In Methods in Molecular Biology (Vol. 1959, pp. 173–183). Humana Press Inc. https://doi.org/10.1007/978-1-4939-9164-8_12
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