Transcription factors RUNX1 and RUNX3 in the induction and suppressive function of Foxp3+ inducible regulatory T cells

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Abstract

Forkhead box P3 (FOXP3) + CD4 + CD25+ inducible regulatory T (iT reg) cells play an important role in immune tolerance and homeostasis. In this study, we show that the transforming growth factor-β (TGF-β) induces the expression of the Runt-related transcription factors RUNX1 and RUNX3 in CD4+ T cells. This induction seems to be a prerequisite for the binding of RUNX1 and RUNX3 to three putative RUNX binding sites in the FOXP3 promoter. Inactivation of the gene encoding RUNX cofactor core-binding factor-β (CBFβ) in mice and small interfering RNA (siRNA)-mediated suppression of RUNX1 and RUNX3 in human T cells resulted in reduced expression of Foxp3. The in vivo conversion of naive CD4+T cells into Foxp3+iT reg cells was significantly decreased in adoptively transferred CbfbF/F CD4-cre naive T cells into Rag2-/- mice. Both RUNX1 and RUNX3 siRNA silenced human T reg cells and CbfbF/F CD4-cre mouse T reg cells showed diminished suppressive function in vitro. Circulating human CD4+CD25 high CD127-T reg cells significantly expressed higher levels of RUNX3, FOXP3, and TGF-β mRNA compared with CD4+CD25- cells. Furthermore, FOXP3 and RUNX3 were colocalized in human tonsil T reg cells. These data demonstrate Runx transcription factors as a molecular link in TGF-β-induced Foxp3 expression in iT reg cell differentiation and function. © 2009 Klunker et al.

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Klunker, S., Chong, M. M. W., Mantel, P. Y., Palomares, O., Bassin, C., Ziegler, M., … Akdis, C. A. (2009). Transcription factors RUNX1 and RUNX3 in the induction and suppressive function of Foxp3+ inducible regulatory T cells. Journal of Experimental Medicine, 206(12), 2701–2715. https://doi.org/10.1084/jem.20090596

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