Protocols are described for the measurement of radioisotopic cation influx and efflux in bacteria using Salmonella as a model system. Methods are discussed for both the use of primary radioisotopes for measurement, e.g., using 54Mn2+ to measure Mn2+ influx, and the use of surrogate radioisotopes when the primary radioisotope is not available, e.g., use of 63Ni2+ or 57Co2+ in place of 28Mg2+ to measure Mg2+ influx. Both vacuum filtration and centrifugation assays are described. In addition, the use and misuse of chelating agents is discussed.
CITATION STYLE
Maguire, M. E. (2007). Magnesium, manganese, and divalent cation transport assays in intact cells. Methods in Molecular Biology (Clifton, N.J.), 394, 289–305. https://doi.org/10.1007/978-1-59745-512-1_14
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