Repeated batch production of theanine by coupled fermentation with energy transfer using membrane-enclosed γ-glutamylmethylamide synthetase and dried yeast cells

Abstract

γ-Glutamylmethylamide synthetase and dried baker's yeast cells were enclosed together in a dialysis membrane tube to produce theanine repeatedly by coupled fermentation with energy transfer. The membrane-enclosed enzyme preparation (M-EEP) formed approximately 600 mm theanine from glutamic acid and ethylamine at a 100% conversion rate. M-EEP maintained its productivity of theanine during six consecutive reactions in a mixture containing NAD +.