Morphological and physiological changes in transgenic Chrysanthemum morifolium Ramat. 'Ogura-nishiki' with rolC

Abstract

The effects of rolC on morphogenesis of Chrysanthemum morifolium Ramat. 'Ogura-nishiki' were studied. The ray flowerets in the rolC-transformed plants were shorter than those in wild-type plants, but the transformation enhanced outgrowth of lateral shoots and reduced internode elongation. Studies on adventitious shoot and root formation from leaf segments revealed that the rolC transgene led to reduced root formation but enhanced adventitious bud formation. This enhanced cytokinin-like activity was consistent with phenotypes observed in the rolC-transformed plants.