We have established a reverse genetics approach for the routine generation of medaka (Oryzias latipes) gene knockouts. A cryopreserved library of N-ethyl-N-nitrosourea (ENU) mutagenized fish was screened by high-throughput resequencing for induced point mutations. Nonsense and splice site mutations were retrieved for the Blm, Sirt1, Parkin and p53 genes and functional characterization of p53 mutants indicated a complete knockout of p53 function. The current cryopreserved resource is expected to contain knockouts for most medaka genes. © 2006 Taniguchi et al.; licensee BioMed Central Ltd.
CITATION STYLE
Taniguchi, Y., Takeda, S., Furutani-Seiki, M., Kamei, Y., Todo, T., Sasado, T., … Cuppen, E. (2006). Generation of medaka gene knockout models by target-selected mutagenesis. Genome Biology, 7(12). https://doi.org/10.1186/gb-2006-7-12-r116
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