Diversity of archaea and bacteria in a biogas reactor fed with Pennisetum sinese Roxb by 16S rRNA sequence analysis

Abstract

Purpose: To investigate the structure and function of the complex rumen microbial community in a biogas reactor by 16S rRNA gene analysis, which was fed with Pennisetum sinese Roxb as the monosubstrate. Methods: Two 16S ribosomal RNA (rRNA) clone libraries of bacteria and archaea were established by polymerase chain reaction. Community structure was determined by phylogenetic analyses of 119 and 100 16S rRNA gene clones from the bacterial and archaeal libraries, respectively. Results: In the bacterial library, 13.4% of clones were affiliated with Treponema porcinum, 5.9% with Eubacterium limosum, 5% with Clostridium, 5% with Bacteroidetes, 4.2% with Firmicutes, 2.5% with Anaerofilum and a total of 64% clones belonged to unclassified or uncultured bacteria. In the archaeal library, Methanobacterium curvum made up 12% of known clones, Methanosarcina barkeri represented 8%, Methanobacterium bryantii represented 4% and Methanofollis ethanolicus represented 2%, respectively; the remaining 74% of the clones were unclassified archaebacteria. Conclusion: T. porcinum and M. curvum are the most predominant bacteria and archaea in a biogas reactor fed with P. sinese as the sole substrate.