The formation of ATP produced from poly(ADP-ribose) [(ADP-R)n] has been suggested to be required to repair damaged DNA. Here we investigate whether this ATP is involved in DNA replication processes during DNA repair. Poly(ADP-ribosyl)ated mid-S phase cell nuclei, which were isolated from synchronized HeLa S3 cells followed by the treatment with a DNA damaging agent, N-methyl-N′-nitro-N-nitrosoguanidine (MNNG), were revealed to retain DNA replication synthesizing activity during preincubation for de-poly(ADP-ribosyl) ation only in the presence of pyrophosphate (PPi) before DNA synthesis was started by adding 3 mM ATP. This DNA replication activity was not maintained in the presence of a potent and specific inhibitor of poly(ADP-ribose) glycohydrolase (PARG), Oenothein B (Oen B) during the preincubation with PPi. In the preincubation with PPi, μM orders of ATP was produced from (ADP-R) n. These results point to an important function of ATP generated from (ADP-R)n in nuclei for the maintenance of replication apparatus during DNA repair. © 2007 Pharmaceutical Society of Japan.
CITATION STYLE
Maruta, H., Okita, N., Takasawa, R., Uchiumi, F., Hatano, T., & Tanuma, S. I. (2007). The involvement of ATP produced via (ADP-ribose)n in the maintenance of DNA replication apparatus during DNA repair. Biological and Pharmaceutical Bulletin, 30(3), 447–450. https://doi.org/10.1248/bpb.30.447
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