Establishing a stable interaction between a T cell and an antigen presenting cell (APC) involves the formation of an immune synapse (IS). It is through this structure that the T cell can integrate all the signals provided by the APC. The IS also serves as a mechanism for TCR downregulation through internalization. Here, we describe methods for visualizing MHC-engaged T cell receptor (TCR) internalization from the IS in human cell lines and mouse primary T cells by confocal fluorescence microscopy techniques.
CITATION STYLE
Calleja, E., Alarcón, B., & Oeste, C. L. (2017). Studying the dynamics of TCR internalization at the immune synapse. In Methods in Molecular Biology (Vol. 1584, pp. 89–99). Humana Press Inc. https://doi.org/10.1007/978-1-4939-6881-7_7
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