HaCaT cells are a spontaneously immortalized, human keratinocyte line that has been widely used for studies of skin biology and differentiation. Under typical culture conditions HaCaT cells have a partially to fully differentiated phenotype due to the high calcium content of both standard media and fetal bovine serum. This chapter describes low-calcium culture conditions for reverting HaCaT cells to the fully basal state followed by subsequent controlled differentiation using calcium induction.
CITATION STYLE
Wilson, V. G. (2013). Growth and Differentiation of HaCaT Keratinocytes (pp. 33–41). https://doi.org/10.1007/7651_2013_42
Mendeley helps you to discover research relevant for your work.