Mapping and Sequence of the Gene Encoding Protein pl7, a Major African Swine Fever Virus Structural Protein

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Abstract

The gene encoding protein p17, a major structural protein of African swine fever virus, has been mapped and sequenced. Protein p17 was purified from dissociated virus by reverse-phase HPLC and the amino acid sequence of a peptide obtained after digestion of protein p17 with cyanogen bromide was determined by automated Edman degradation. To map the gene encoding protein p17, a mixture of 17-mer oligonucleotides based upon a part of the amino acid sequence was hybridized to cloned African swine fever virus DNA restriction fragments. This allowed the location of the gene in the fragment EcoRI D of the viral genome. The nucleotide sequence of a Sa/I/Kpnl fragment revealed an open reading frame designated D117R encoding a protein of 117 amino acids with a deduced molecular mass of about 13,000 Da. A transcriptional analysis revealed that the p17 gene is expressed late in the viral infection cycle. © 1995 Academic Press. All rights reserved.

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Simón-Mateo, C., Freue, J. M. P., Andrés, G., López-Otin, C., & Vinuela, E. (1995). Mapping and Sequence of the Gene Encoding Protein pl7, a Major African Swine Fever Virus Structural Protein. Virology, 206(2), 1140–1144. https://doi.org/10.1006/viro.1995.1039

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