In vitro angiogenesis assay: Endothelialmigration, proliferation, and tube formation

Abstract

Basic in vitro angiogenesis assays conducted on endothelial cells of various origin are critically important in allowing us to identify mechanisms responsible for modulation of vascularization during pathological events and disease. In this chapter, we review methods for and measurement of proliferation, migration and tube-like structure formation in cerebral endothelial cultures. Alternative protocols, materials required to conduct these assays, and example results are also provided. Accurate reproducibility of these assays is highly important and therefore we have provided a section on trouble shooting near to the end. The assays are applicable to endothelial cells of any origin with minor modifications and optimizations.