Detection of Escherichia Albertii in Urinary and Gastrointestinal Infections in Kermanshah, Iran

Abstract

Background: Escherichia albertii has been recently isolated from the feces of people with gastroenteritis as a pathogen that causes diarrhea. Due to insufficient information on the phenotypic and biochemical characteristics of E. albertii, it is difficult to distinguish it from other species of the Enterobacteriaceae family and, therefore, it is mistakenly identified as Escherichia coli or even Hafnia alvei. Objective: The present study which was conducted for the first time in Iran aimed to identify E. albertii in samples from individuals afflicted with urinary and gastrointestinal infections by using the polymerase chain reaction (PCR) method. The required samples were obtained from clinical laboratories in Kermanshah. Materials and Methods: Firstly, a total of 60 urinary and 40 fecal samples identified as E. coli in clinical laboratories were re-evaluated in terms of specific phenotypic and biochemical characteristics of E. coli. Then, two lysP and mdh genes were detected for E. albertii, and the uidA gene was found for E. coli by PCR using specific primers pairs. Results: The results from phenotypic and biochemical tests indicated that all samples shared common characteristics with E. coli. However, PCR findings demonstrated that out of 100 samples, 6 samples (6%) contained specific genes of E. coli while 94 remaining samples (94%) showed the uidA gene. Out of the given 6 samples, 5 samples carried urinary tract infections and only one showed gastrointestinal infection. Conclusion: Our study findings revealed that E. albertii could have been considered as one of the causes for urinary and gastrointestinal infections in Iran, and that it was mistakenly identified as E. coli in clinical laboratories.