X-ray diffraction experiments on protein crystals are at the core of the structure determination process. An overview of X-ray sources and data collection methods to support structure-based drug design (SBDD) efforts is presented in this chapter. First, methods of generating and manipulating X-rays for the purpose of protein crystallography, as well as the components of the diffraction experiment setup are discussed. SBDD requires the determination of numerous protein-ligand complex structures in a timely manner, and the second part of this chapter describes how to perform diffraction experiments efficiently on a large number of crystals, including crystal screening and data collection. © 2012 Springer Science+Business Media, LLC.
CITATION STYLE
Snell, G. (2012). X-ray sources and high-throughput data collection methods. Methods in Molecular Biology, 841, 93–141. https://doi.org/10.1007/978-1-61779-520-6_5
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