On the basis of pIP501, a green fluorescent protein (GFP)-tagged monitoring tool was constructed for quantifying plasmid mobilization among Gram-positive bacteria and between Gram-positive Enterococcus faecalis and Gram-negative Escherichia coli. Furthermore, retromobilization of the GFP-tagged monitoring tool was shown from E. faecalis OG1X into the clinical isolate E. faecalis T9. © 2012, American Society for Microbiology.
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Arends, K., Schiwon, K., Sakinc, T., Hübner, J., & Grohmann, E. (2012). Green fluorescent protein-labeled monitoring tool to quantify conjugative plasmid transfer between Gram-positive and Gram-negative bacteria. Applied and Environmental Microbiology, 78(3), 895–899. https://doi.org/10.1128/AEM.05578-11