The development of expressed protein ligation (EPL) widened the scope of questions that could be addressed by mechanistic biochemistry. Protein trans-splicing (PTS) relies on the same basic chemical principles, but utilizes split inteins to tracelessly ligate distinct peptide or polypeptide fragments together with native peptide bonds. Here we present a method to adapt PTS methodologies for their use in live cells, in order to deliver synthetic or native histone modifications. As an example, we provide a protocol to incorporate a small molecule fluorophore into chromatinized histones. The protocol should be easily adaptable to incorporate other modifications to chromatin in vivo.
CITATION STYLE
Prescott, N. A., & David, Y. (2020). In Vivo Histone Labeling Using Ultrafast trans-Splicing Inteins. In Methods in Molecular Biology (Vol. 2133, pp. 201–219). Humana Press Inc. https://doi.org/10.1007/978-1-0716-0434-2_10
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