Inhibition of gastrulation in Xenopus embryos by an antibody against a cathepsin L-like protease

Abstract

An antibody against cathepsin L-like protease (AACLP) was injected into one cell of 2-celled Xenopus embryos. The blastopores of AACLP-injected embryos either did not invaginate or failed to complete invagination. As a result of this failure to complete gastrulation, the body axes could not form normally and tail bud stage embryos were bent dorsally. Embryos injected with a control antibody (CA) developed normally through the tadpole stage. Mesodermal induction was not inhibited in embryos exhibiting this AACLP- induced gaslrulation defect, but the mesodermal structure of these embryos was organized incorrectly due to the defective gastrulation during the early stages.