In a “sandwich” enzyme-linked immunosorbent assay (ELISA) designed to detect an antigen in a complex protein mixture, the antigen is usually captured via an antibody adsorbed to the wells of a microplate. Plate preparation for standard assay involves a passive adsorption of capture antibodies followed by the incubation of blocking agents. Here, we describe a new strategy that replaces these two time-consuming adsorption steps (up to 15 h) by a unique step corresponding to the covalent grafting of the capture antibody on a carboxymethylated dextran (CMD) layer, a single step completed in 15 min. Taking advantage of the CMD low-fouling properties, blocking agent-free buffer solutions can be used as diluent in the improved approach.
CITATION STYLE
Liberelle, B., Fortier, C., & De Crescenzo, G. (2014). Enhanced ELISA based on carboxymethylated dextran coatings. Methods in Molecular Biology, 1172, 39–47. https://doi.org/10.1007/978-1-4939-0928-5_4
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