Many chemotaxis assays allow for the assessment of bacterial chemotaxis by determining the number of cells migrating toward a chemoattractant or away from a chemorepellent. Some of these assays use a capillary filled with a chemoeffector/agarose mixture to allow cells to accumulate at the mouth of the capillary. Subsequently, assumptions about the relative strengths of chemotaxis strength are based on visual comparisons. Here, we describe a modification of this assay that uses a hydrogel matrix to enable quantitative time-course measurements by analyzing image pixel intensities. This approach allows a high-throughput method when coupled with the aid of a motorized microscope stage.
CITATION STYLE
Webb, B. A., Arapov, T. D., & Scharf, B. E. (2018). Quantification of bacterial chemotaxis responses at the mouths of hydrogel capillaries. In Methods in Molecular Biology (Vol. 1729, pp. 33–45). Humana Press Inc. https://doi.org/10.1007/978-1-4939-7577-8_4
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