Topical application of recombinant platelet-derived growth factor increases the rate of healing and the level of proteins that regulate this response

20Citations
Citations of this article
28Readers
Mendeley users who have this article in their library.

Abstract

A bipedicle ischaemic rat skin flap model was used to study the effects of daily topical applications of platelet-derived growth factor (PDGF) on the healing of ischaemic wounds. Levels of tumour necrosis factor-alpha (TNFA), interleukin 1-beta (IL1B) and both the latent and active forms of matrix metalloproteinase 2 (MMP2) and 9 (MMP9) were measured. Full-thickness wounds were made on a total of 72 adult male Sprague-Dawley rats. Each group of 18 rats with normal and ischaemic wounds received either vehicle or 0·01% recombinant PDGF-BB. Additional applications were made on the wounds on a daily basis. Wound areas were measured at 0, 1, 3, 5, 7 9 and 13days after wounding. Ischaemia caused a delay in wound healing as well as an increase in TNFA, IL1B and both the pro and active forms of MMP2 and MMP9. PDGF accelerated the rate of wound healing in both normal and ischaemic wounds and negated the effect of ischaemia. PDGF reduced the TNFA concentration in both normal and ischaemic wounds, and the rate of wound healing closely resembled the pattern of TNFA protein expression. PDGF also reduced both the magnitude and duration of the increases in IL1B and both the pro and active forms of MMP2 and MMP9 induced by ischaemia.

References Powered by Scopus

Acute-phase proteins and other systemic responses to inflammation

5591Citations
N/AReaders
Get full text

Cutaneous wound healing

5125Citations
N/AReaders
Get full text

Wound healing - Aiming for perfect skin regeneration

4072Citations
N/AReaders
Get full text

Cited by Powered by Scopus

Role of growth factors and cytokines in diabetic foot ulcer healing: A detailed review

220Citations
N/AReaders
Get full text

Skin substitutes for acute and chronic wound healing: an updated review

175Citations
N/AReaders
Get full text

Recent advances in decellularized biomaterials for wound healing

65Citations
N/AReaders
Get full text

Register to see more suggestions

Mendeley helps you to discover research relevant for your work.

Already have an account?

Cite

CITATION STYLE

APA

Gowda, S., Weinstein, D. A., Blalock, T. D., Gandhi, K., Mast, B. A., Chin, G., & Schultz, G. S. (2015). Topical application of recombinant platelet-derived growth factor increases the rate of healing and the level of proteins that regulate this response. International Wound Journal, 12(5), 564–571. https://doi.org/10.1111/iwj.12165

Readers' Seniority

Tooltip

PhD / Post grad / Masters / Doc 12

60%

Researcher 5

25%

Professor / Associate Prof. 2

10%

Lecturer / Post doc 1

5%

Readers' Discipline

Tooltip

Medicine and Dentistry 9

45%

Agricultural and Biological Sciences 5

25%

Engineering 4

20%

Veterinary Science and Veterinary Medic... 2

10%

Save time finding and organizing research with Mendeley

Sign up for free