Microbial degradation of disinfectants. A new chlorhexidine degradation intermediate (CHDI), CHDI-C, produced by Pseudomonas sp. Strain No. A-3

Abstract

To clarify the degradation pathway of chlorhexidine by a microbe, Pseudomonas sp. Strain No. A-3, the isolation and identification of microbial chlorhexidine degradation products were attempted. A new chlorhexidine degradation intermediate (CHDI), named CHDI-C, was isolated by extraction with ethylacetate, n-butanol, column chromatography using Silica gel, and purified by preparative thin layer chromatography. The chemical structure of this product was examined by infrared, 1H NMR, 13C NMR and fast atom bombardment mass spectra studies. Based on the spectroscopic data, this product was assumed to be a modified compound of chlorhexidine (molecular weight, MW; 530). From the proposed structure, CHDI-C was assumed to be a new chlorhexidine degradation intermediate.