Association of Rab1B with GDP-dissociation inhibitor (GDI) is required for recycling but not initial membrane targeting of the rab protein

Abstract

We have identified a Rab1B effector-domain mutant (D44N) that, when geranylgeranylated by Rab:geranylgeranyltransferase (GGTase II) in cell-free systems or intact cells, fails to farm detectable complexes with GDP- dissociation inhibitors (GDIs). GDI-Rab complexes were collected on anti- FLAG affinity beads after incubating recombinant geranylgeranylated Rab1B with FLAG epitope-tagged GDI in vitro, or transiently coexpressing Myc- tagged Rab1B with FLAG-GDI-α or FLAG-GDI-2 in human embryonal kidney 293 cells. [3H]Mevalonate labeling and immunoprecipitation studies confirmed that the inability of Myc-Rab1B(D44N) to associate with GDI in vivo was not due to failure of the mutant to undergo geranylgeranylation. Immunofluorescence localization and immunoblot analysis of subcellular fractions indicated that expressed Myc-Rab1B(D44N) was efficiently delivered to intracellular membranes in 293 cells. This was confirmed when the fate of the prenylated pool of gab1B(D44N) in 293 cells was traced by labeling the geranylgeranyl groups attached to the nascent protein with [3H]mevalonate. However, in contrast to the prenylated RabB(WT), which was distributed in both the membrane and soluble fractions, the prenylated Rab1B(D44N) was completely absent from the cytosol. Overexpression of Myc-Rab1B(D44N) did not impair ER → Golgi glycoprotein trafficking in 293 cells, which was assessed by monitoring the Golgi-dependent processing of coexpressed β-amyloid precursor protein. The current findings suggest that nascent prenylated Rab1B can be delivered to intracellular membranes in intact cells without forming a stable complex with GDI, but that recycling of prenylated Rab1B to the cytosolic compartment is absolutely dependent on GDI interaction.