Enhanced epiphytic coexistence of near-isogenic salicylate-catabolizing and non-salicylate-catabolizing Pseudomonas putida strains after exogenous salicylate application

Abstract

The hypothesis that epiphytic bacterial populations can coexist through nutritional resource partitioning was tested with the near-isogenic bacterial strain pair Pseudomonas putida R20 and R20(pNAH7). The plasmid pNAH7 conferred upon R20 the ability to catabolize salicylate as a sole carbon source in vitro. P. putida R20(pNAH7) also catabolized exogenously applied salicylate in planta and reached a significantly larger epiphytic population size than the near-isogenic parental strain R20 under the same conditions. This supports previous observations that epiphytic populations on plants grown under nitrogen-sufficient conditions are limited by carbon availability. In the absence of exogenous salicylate, R20 and R20(pNAH7) competed for and partitioned endogenous carbon according to their inoculum proportion in replacement series experiments, exhibiting a low level of coexistence. In the presence of exogenous salicylate, however, R20(pNAH7) was solely able to catabolize the additional carbon and achieved a higher level of coexistence with R20 than was possible in the absence of exogenous carbon.