Tracing paternal ancestry in mice, using the Y-linked, sex-determining locus, Sry

Abstract

The molecular evolution of mammalian Y-linked DNA sequences is of special interest because of their unique mode of inheritance: most Y-linked sequences are clonally inherited from father to son. Here we investigate the use of Y- linked sequences for phylogenetic inference. We describe a comparative analysis of a 515-bp region from the male sex-determining locus, Sry, in 22 murine rodents (subfamily Murinae, family Muridae), including representatives from nine species of Mus, and from two additional murine genera-Mastomys and Hylomyscus. Percent sequence divergence was <0.01% for comparisons between populations within a species and was 0.19%-8.16% for comparisons between species. Our phylogenetic analysis of 12 murine taxa resulted in a single most-parsimonious tree that is highly concordant with phylogenies based on mitochondrial DNA and allozymes. A total evidence tree based on the combined data from Sry, mitochondrial DNA, and allozymes supports (1) the monophyly of the subgenus Mus, (2) its division into a Palearctic group (M. musculus, M. domesticus, M. spicilegus, M. macedonicus, and M. spretus) and an Oriental group (M. cookii, M. cervicolor, and M. caroli), and (3) sister-group relationships between M. spicilegus and M. macedonicus and between M. cookii and M. cervicolor. We argue that Y-chromosome DNA sequences represent a valuable new source of characters for phylogenetic inference.